The Williams sisters were eliminated by the Czech pair of Lucie Hradecka and Linda Noskova 7-6 (5), 6-4 at Flushing Meadows.
Arthur Ashe Stadium had never hosted a first-round doubles match — for women or men, during the night or day — until this one featuring two members of one family who have combined to claim 14 Grand Slam titles in doubles.
The Williams sisters, who did not do interviews after the match, were partnering up for the first time since the 2018 French Open. This was their fourth first-round doubles defeat at a Slam; the most recent had been at the 2013 French Open.

